HPLC Column | Agilent HC-C18(2) Small Molecule Separations LC Column  

Agilent HC-C18(2) columns are an excellent choice for separations of many types of compounds.
Agilent HC-C18(2) is a more retentive C18 with a higher carbon load. An excellent value alternative to other high carbon load columns, it also provides superior peak shape for basic compounds.
Features Of Agilent HC-C18(2) Small Molecule Separations LC Column:
Higher carbon load - 17% - provides greater retention for moderately polar and non-polar compounds
Ideal for non-polar compounds and separations that start at mid-level % organic (at least greater than 10% organic)
Good choice for industrial samples or samples dissolved in organic/mostly organic solvents
Stable over a very wide pH range (2-9) for maximum flexibility
To compare technical information and specifications for different pha

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HPLC Column | Agilent Prep Small Molecule Separations LC Column  

[caption id="" align="aligncenter" width="475"] Agilent Prep Small Molecule Separations LC Column[/caption]
Agilent Prep LC Columns are designed for high loadability to purify milligram to gram quantities of products. Column dimensions are available for most preparative sample types ranging from high throughput samples generated in drug discovery to lab scale prep and up to some pilot scale sample preparation. Preparative sized columns are available in 21.2, 30 and 50 mm internal diameters with lengths ranging from 50–250 mm. Columns are available in 5 and 10 µm particle sizes with very high efficiency in every dimension.
Features Of Agilent Prep Small Molecule Separations LC Column:
High loadability for maximum sample purification
Easy scalability from 4.6 mm id to 50 mm id for rapid

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HPLC Column | Agilent Load & Lock Small Molecule Separations LC Column  

Agilent offers a complete range of Load & Lock column systems for laboratory and process preparative LC. They are designed to enable users to easily and quickly pack their own preparative high efficiency columns. This is the right solution for applications ranging in scale from discovery (milligrams) to production (multi-kilos) of pharmaceutical compounds, peptides, and natural products. Our Load & Lock columns have a unique fluid/sample distribution system to maximize productivity. It is the only system that provides dynamic axial compression (DAC) and static "locked" axial compression (SAC) and is designed for easy operation to deliver greater convenience.
Features Of Agilent Load & Lock Small Molecule Separations LC Column:
Mobile packing station supports three different co

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HPLC Column | Agilent MicroBore Small Molecule Separations LC Column  

MicroBore (1 mm id) columns are a good choice when sample sizes are limited. They will improve detection limits 5 times over 2.1 mm id columns when the same sample mass is used. This increase in sensitivity can be critical. MicroBore columns use low flow rates (typically ~50 µL/min). Therefore, these columns are ideal for use with detectors requiring low flow ratessuch as some mass spectrometers. MicroBore columns perform optimally with HPLC systems purchased or modified for microbore use. Guard columns are now available with an adjustable tube stop depth to provide a perfect zero dead volume connection every time.
Features Of Agilent MicroBore Small Molecule Separations LC Column:
High sensitivity for small sample sizes
Compatible with LC/MS interfaces
Wide variety of bonded phases

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HPLC Column | Agilent ChiraDex Small Molecule Separations LC column  

Applications: Barbiturates, benzothioadiazines, calcium antagonists, PTH-amino acids
Features Of Agilent ChiraDex Small Molecule Separations LC column:
For routine separation of enantiomers
Available as ChiraDex cartridge columns
Novel manufacturing process bonds ß-cyclodextrin to spherical 5-um silica gel by means of a chemical spacer
Enantiomeric separations have been achieved with ChiraDex using simple nonchiral solvent systems

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HPLC Column | Agilent ZORBAX Extend-C18 Small Molecule Separations LC Column  

Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5. Columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH.
Features Of Agilent ZORBAX Extend-C18 Small Molecule Separations LC Column:
High efficiency and long life at high pH - up to pH 11.5
Unique bidentate bonding and double endcaping provides high pH stability
More efficiency and better peak shape than polymer-based columns
Improve retention, resolution and peak shape of basic compounds
High sensitivity for LC/MS sepa

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HPLC Column | Agilent ZORBAX Eclipse XDB-CN Small Molecule Separations LC Column  

For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings.
Made from highly pure Rx-SIL
Excellent choice for normal-phase applications with basic compounds
Equilibrates more rapidly than ZORBAX Rx-Sil and is used for many of the same normal-phase applications
To compare technical information and specifications for different phases, see our phase specifications chart.

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HPLC Column | Agilent LiChrospher Small Molecule Separations LC Column  

Agilent LiChrospher silica columns are made with spherical, 'sil' type porous silica particles. LiChrospher 60 RP-select B starts with 60Å pore size silica optimized for symmetrical peak shapes of basic compounds. LiChrospher 100Å is offered in both C8 and C18 as well as an endcapped C18. These columns are noted for high sample capacity and efficiency. The popular LiChrospher packings are offered in the convenient and economical Agilent cartridge configuration. For normal phase chromatography, Agilent supplies polar modified silica gel phases LiChrospher Si, LiChrospher NH2 , LiChrospher DIOL and LiChrospher CN as convenient cartridge columns.
Features Of Agilent LiChrospher Small Molecule Separations LC Column:
Available in 5 µm particle size
Reversed-phase available in RP-C18, RP-18

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HPLC Column | Agilent ZORBAX PrepHT Small Molecule Separations LC Column  

ZORBAX optimized phases are now available in preparative dimensions as PrepHT columns. These columns are 21.2 mm and larger in diameter with 5 µm and 7 µm particles to provide high resolving power. They are also available in various lengths to allow optimum resolving power and speed of analyses. In preparative chromatography, it is important to obtain high purity and high recovery along with high throughput. Agilent ZORBAX PrepHT columns have a broad range of selectivities to achieve these objectives.
Features Of Agilent ZORBAX PrepHT Small Molecule Separations LC Column:
Easy scale-up from analytical to preparative scale with ZORBAX phases
Fast preparative separations, up to 2000 mg
5 to 7 μm particles for high efficiency and high yield
Easy to install finger-tight connections seal

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HPLC Column | Agilent ZORBAX Eclipse Amino Acid Analysis (AAA) LC Column  

The ZORBAX Eclipse AAA high efficiency HPLC column rapidly separates amino acids following an updated and improved protocol for use with the Agilent 1100 HPLC. Total analysis from injection to injection can be achieved in as little as 14 min (10 min analysis time) on the short, 7.5 cm column and 24 min (16 min analysis time) on the 15 cm column length. Exceptional sensitivity (5 - 50 pmol with DAD,FLD) and reliability are achieved using both OPA and FMOC derivitization chemistries in one fully automated procedure using the Agilent 1100 HPLC instrument. Eclipse AAA columns are used for high resolution rapid analysis of 24 amino acids. The Eclipse AAA is use tested for amino acid analysis (AAA). The Eclipse AAA uses well known OPA and FMOC derivatization techniques.
Features Of  Agilent ZOR

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HPLC Column | Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column  

ZORBAX Carbohydrate Analysis Columns are reproducible, efficient, and flexible. These columns use ZORBAX porous silica microsphere technology. Silica manufacture, bonding and packing are all performed in our ISO9001 facilities. ZORBAX Carbohydrate Analysis Columns can handle high volume injections as much as 50 µL on a 4.6 x 150 mm column.
Features Of Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column:
Efficient – uses ZORBAX porous silica microsphere technology; silica manufacturing, bonding and packing are all performed in Agilent's ISO 9001 facilities
Flexible – can handle high volume injections – as much as 50 μL on a 4.6 x 150 mm column
Recommended for use with refractive index detectors (RID)

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Notes on Cleaning bound Protein from RP HPLC columns  

First, a few comments:

·         Before proceeding with any column regeneration or cleaning procedures, always refer to the specific advice provided by the column manufacturer. Approved maintenance and cleaning instructions can often be found in the product guide which comes with the new column. Their guidelines supersede these!

·         Columns are consumable items. After a suitable amount of use, the time and materials required to regenerate them may cost more than the purchase of a replacement column. Always have a new, spare column on hand.

·         Protect your detector. Before washing or cleaning the column, disconnect the column outlet line and direct the column to waste only.

For RP supports, if buffers have been used, always start by washi

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clogged column frit HPLC problem  

Leaks are a common problem in HPLC analyses. To minimize leaks in your system, avoid interchanging hardware and fittings from different manufacturers. Incompatible fittings can be forced to fit initially, but the separation may show problems and repeated connections may eventually cause the fitting to leak. If interchanging is absolutely necessary, use appropriate adapters and check all connections for leaks before proceeding. Highly concentrated salts (>0.2 M) and caustic mobile phases can reduce pump seal efficiency. The lifetime of injector rotor seals also depends on mobile phase conditions, particularly operation at high pH. In some cases, prolonged use of ion pair reagents has a lubricating effect on pump pistons that may produce small leaks at the seal. Some seals do not perform

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Mobile Phase (eluent) In Column Chromatography  

The mobile phase or eluent is either a pure solvent or a mixture of different solvents. It is chosen so that the retention factor value of the compound of interest is roughly around 0.2 - 0.3 in order to minimize the time and the amount of eluent to run the chromatography. The eluent has also been chosen so that the different compounds can be separated effectively. The eluent is optimized in small scale pretests, often using thin layer chromatography (TLC) with the same stationary phase.
There is an optimum flow rate for each particular separation. A faster flow rate of the eluent minimizes the time required to run a column and thereby minimizes diffusion, resulting in a better separation. However, the maximum flow rate is limited because a finite time is required for the analyte to equi

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Stationary Phase In Column Chromatography  

The stationary phase or adsorbent in column chromatography is a solid. The most common stationary phase for column chromatography is silica gel, the next most common being alumina. Cellulose powder has often been used in the past. Also possible are ion exchange chromatography,reversed-phase chromatography (RP),affinity chromatography or expanded bed adsorption (EBA). The stationary phasesare usually finely ground powders or gels and/or are microporous for an increased surface, though in EBA a fluidized bed is used.
There is an important ratio between the stationary phase weight and the dry weight of the analyte mixture that can be applied onto the column. For silica column chromatography, this ratio lies within 20:1 to 100:1, depending on how close to each other the analyte components are

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What Is Column chromatography?  

For more details on this topic, see Column chromatography.
Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample.
In 1978, W. Clark Still introduced a modified version of column chromatography called flash column chromatography (flash).The technique is very similar to the traditional column chromatography, except for that the

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Column Chromatography is the the first form of techniques developed in chromatography!  

Column chromatography is the prototype or the basic type of chromatography. It was the first form of techniques developed in chromatography. One can easily explain the principle and procedure of chromatography using it.
Other types of chromatography techniques like high performance liquid chromatography (HPLC), gas chromatography (GC), paper chromatography were developed with column chromatography as a module and making slight variations.
In-spite of many advanced methods of chromatography, still this method of chromatography is widely used in research and industry.
This chromatography is basically a type of adsorption chromatography techniques.

Here the separation of components depends upon the extent of adsorption to stationary phase. Here the stationary phase is a polar solid materia

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column chromatography principle  

When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates.Those with lower affinity and adsorption to stationary phase move faster and eluted out first while those with greater adsorption affinity move or travel slower and get eluted out last.
The solute molecules adsorb to the column in a reversible manner. The rate of the movement of the components is given as follows
R= Rate of movement of a component / Rate of movement of mobile phase. i.e. it is the ratio of distance moved by solute to the distance moved by solvent.

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Principle Of Column Chromatography  

Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to recycling.
The classical preparative chromatography column is a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter (a glass frit or glass wool plug – to prevent the loss of the stationary phase) at the bottom. Two methods are generally used to prepare a column: the dry method and the wet method.

For the dry

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