HPLC Chemiluminescent Nitrogen Detector (CLND)

Similar to FL, but instead of using a light source to excite the analyte atoms, the excitation is initiated by chemical reaction. Since it is not relied on the external excitation source, the noise is small, results in high signal to noise ratio, i.e. it provides even higher sensitivity than FL.

The Chemiluminescent Nitrogen Detector (CLND) for use with high-performance liquid chromatography (HPLC) allows for the low-level detection of nitrogen-containing compounds with simple quantitation. The nitrogen selective detector’s equimolar response (i.e., equal response for nitrogen independent of its chemical environment) allows for any nitrogen-containing compound to be quantitated as long as the number of nitrogens are known. The HPLCCLND provides a new detection method for analytes that are not available in large quantities or have unknown chemical or physical characteristics such as oxidation products, metabolites, or impurities. Ethoxyquin is a primary antioxidant that is used to preserve many food products and animal feeds. HPLCCLND is used in the study of the oxidation products of ethoxyquin because limited quantities of these compounds are available and subsequent calibration curves are difficult to maintain. HPLCCLND as a new method of detection has been evaluated for its equimolarity of response, linear range, limit of detection, and limit of quantitation.

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