Shimadzu RF-20A/RF-20Axs Fluorescence LC Detector

Shimadzu RF-20A/RF-20Axs Fluorescence LC Detector

The RF-20A/20AXS fluorescence detectors offer world-leading sensitivity and ease-of-maintenance. The RF-20AXS is a high-sensitivity model that incorporates a temperature-controlled cell with a cooling function as standard.

  • RF-20A / Standard model:

The RF-20A, which offers best-in-class sensitivity, features a water Raman S/N ratio of at least 1200, as well as excellent ease-of-use with such features as maintenance from the front panel and adoption of a long-life lamp.

  • RF-20AXS / Achieves World-Leading Sensitivity :

Offering world-class levels of sensitivity and easy maintenance, the RF-20AXS features a water Raman S/N ratio of at least 2000 and a temperature-controlled cell with a cooling function. This maintains a constant detector cell temperature, even if the room temperature fluctuates significantly, to ensure superb reproducibility with no drop in sensitivity. In addition, the RF-20AXS incorporates an automatic wavelength accuracy check function using an internal low-pressure mercury lamp to provide simple confirmation of the wavelength accuracy for validation.

Achieves World-Leading Sensitivity:

A powerful tool for the detection of ultra-trace components, the RF-20Axs achieved a 21.5 S/N ratio for an injection of 10.48 fg anthracene. This is equivalent to an approx. 1.5 fg detection limit (S/N ratio = 3) and indicates superb sensitivity.

Cell Temperature Control Further Enhances Reproducibility (RF-20AXS) :

The fluorescence intensity drops as the temperature rises. A fluctuation of about 1°C near room temperature may result in approximately 5% intensity fluctuations for some compounds. To prevent this, the RF-20AXS features a temperature-controlled cell with a cooling function. It maintains a constant detector cell temperature, even if the room temperature fluctuates significantly, to ensure superb reproducibility with no drop in sensitivity.

Achieves World-Leading Sensitivity :

A powerful tool for the detection of ultra-trace components, the  RF-20Axs achieved a 21.5 S/N ratio for an injection of 10.48 fg anthracene. This is equivalent to an approx. 1.5 fg detection limit (S/N ratio = 3) and indicates superb sensitivity.

Cell Temperature Control Further Enhances Reproducibility (RF-20AXS) :

The fluorescence intensity drops as the temperature rises. A fluctuation of about 1°C near room temperature may result in approximately 5% intensity fluctuations for some compounds. To prevent this, the RF-20AXS features a temperature-controlled cell with a cooling function. It maintains a constant detector cell temperature, even if the room temperature fluctuates significantly, to ensure superb reproducibility with no drop in sensitivity.

Easy Maintenance :

The Xenon lamp and flow cell can be replaced at the front panel. No positional adjustment is required when replacing the Xenon lamp, and no tools are required to replace the flow cell. The standard flow cell or semimicro flow cell can be rapidly switched. In addition, the Xenon lamp life has been extended to 2000 hours, four times longer than previous Shimadzu lamps.

Support for Ultra Fast Analysis :

  • Switch from Conventional LC to Ultra Fast LC

Fast response is required to follow the sharp peaks obtained in ultra fast LC analysis. The 10 ms response of the RF-20A/20AXS permits ultra fast LC analysis with no loss of separation. In this analysis example, the analysis time was reduced by a factor of more than three, while maintaining the separation.

  • Multi-Component, High-Sensitivity UFLC Analysis

The highly sensitive simultaneous analysis of multiple components requires detection at the optimal wavelengths. The RF-20A/20AXS permit ultra fast, high-sensitivity multi-component analysis using wavelength switching by time program.

Support for Improved Quantitative Analysis Accuracy :

  • Utility of Four-Wavelength Measurement Function

Using detection at a single wavelength when performing multicomponent simultaneous analysis of components with different optimal detection wavelengths necessitates sacrificing sensitivity for certain components. The RF-20A/20AXS detectors eliminate this issue by incorporating a four-wavelength measurement function that permits detection of each component at the optimal wavelength. Detection using wavelength switching in the left-hand diagram exhibits incomplete separation in area (1) and one peak of reduced size in area (2). In such a case, setting up to four optimal wavelengths enhances the quantitative analysis accuracy by reducing the effects of adjacent peaks and improving sensitivity.